EcoRI Type II restriction enzyme subtype: P

Different enzyme:

Recognition Sequence:    help? G^AATTC 5' .. 3' .. G C A T A T T A T A C G .. 3' .. 5'

REBASE Enz Num 993   entered Jan 1 1972 ... modified Jul 3 2014

Acronym: EcoR Prototype: EcoRI Org #: 1394 Organism: Escherichia coli RY13 Organism type: plasmid Organism source: R.N. Yoshimori Growth Temperature: 37 ° Experimental Evidence: biochemistry Exhibits star activity Single-stranded cleavage: y Enzyme gene cloned Enzyme gene sequenced Crystal data present Kinetics data present Molecular Weight: 31057

# sites on Adeno2: 5 Lambda: 5 pBR322: 1 PhiX174: 0 SV40: 1

Status of methylation sensitivity testing... Site frequency in sequenced genomes... DNA RNA hybrids... RNA duplexes... Single stranded DNA... Combined report...

Related Enzymes:      M.EcoRI Related References      sorted by date    in new window      sorted by authors  in new window Commercially Available... Similar enzymes... Sequence Data... Crystal Data...  (8 structures) Kinetic Data...  (5 records) Methylation Sensitivity...

07/9/2017 Dr. Richard J. Roberts and Dana Macelis http://rebase.neb.com/rebase/enz/EcoRI.html